Computational protocol: Meloxicam ameliorates the cartilage and subchondral bone deterioration in monoiodoacetate-induced rat osteoarthritis

Similar protocols

Protocol publication

[…] The study obtained the approval of the Ethics Committee of the University of Medicine and Pharmacy Targu-Mures (no.132/23.12.2013). The sample size was calculated by simulations assuming different means and slightly different variability of the two major histological scores and IL-6 levels between the groups, setting α = 0.05 and 1-β 0.8. Initially, 46 healthy adult male Wistar rats (age: six months, body weight: 170–230 g), obtained from the University Biobase, were entered into the study. The animals were kept under controlled conditions (temperature 22.5 ± 2 °C, humidity 55 ± 5%) on a 12-h light-dark cycle, 5–6 animals per cage (solid bottom), and fed with standard rat chow and water ad libitum. All procedures and experimental protocols used in this study were in compliance with the 2010/63/EU Directive of the European Parliament and Council accepted at September 22, 2010, for the protection of animals used for scientific purposes. [...] The statistical analysis of data was performed with GraphPad Prism 7.01 (GraphPAd Software Inc., La Jolla, CA, USA) and STATISTICA 5.0 (Statsoft, Tulsa, OK, USA). The distribution of histological scores was analysed by use of the Lilliefors and the Shapiro–Wilk test and showed abnormality without exception; therefore, we performed non-parametric tests in interpretation: the Kruskal–Wallis ANOVA test for multiple and the Mann–Whitney U test (two-tailed) for between-group comparison, the Spearman rank correlation for correlation analysis and the Wilcoxon matched pairs test for matched data series (logarithmically transformed cytokine levels). The threshold of significance was set to p = 0.05, and for each test we also calculated the effect size (z, r in the Mann–Whitney U test, r in Spearman correlation). We applied Holm’s sequential Bonferroni adjustment to the p values obtained in multiple comparisons of the osteoarthritic groups (GrpP, GrpM Lo, GrpM Hi). […]

Pipeline specifications

Software tools Biobase, Statistica
Applications Miscellaneous, CAGE analysis
Organisms Rattus norvegicus
Diseases Bone Diseases, Osteoarthritis