Computational protocol: Ready Access to Proquinazid Haptens via Cross-Coupling Chemistry for Antibody Generation and Immunoassay Development

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Protocol publication

[…] Eight-point standard curves, including a blank, were prepared by five-fold serial dilution in PBS from a 1 g/L proquinazid stock solution. Experimental values were fitted to a four-parameter logistic equation using the SigmaPlot software package from SPSS Inc. (Chicago, IL, USA). Assay sensitivity was defined as the concentration of analyte at the inflection point of the fitted curve, typically corresponding to a 50% inhibition (IC50) of the maximum absorbance (Amax) provided that the background signal approaches to zero. […]

Pipeline specifications

Software tools SigmaPlot, SPSS
Application Miscellaneous
Diseases Oculocerebrorenal Syndrome