Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing, Genome binding/occupancy profiling by high throughput sequencing
Number of samples: 20
Release date: Dec 14 2018
Last update date: Jan 23 2019
Access: Public
Dataset link H3K27 tri-methyltransferases CLF and SWN redundantly buffer ABA-induced senescence

Experimental Protocol


Arabidopsis thaliana seedlings were grown on 1/2 Murashige and Skoog (MS) medium without sucrose (2% agar, PH 5.8) under LDs conditions. clf-50swn-1 is in the Ws background. For ABA treatment, 10-d old seedlings were transferred onto 1/2 MS medium containing 50 uM ABA or 100 uM ABA. After 4 days' treatment, the aerial parts were harvested, either frozen in liquid nitrogen for RNA isolation or directly vacuum-infiltrated with formaldehyde crosslinking solution for ChIP assay.

Repositories


GEO

GSE98301

ENA

SRP105367

BioProject

PRJNA384645

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Contact


yijing zhang