|Dataset type:||Expression profiling by high throughput sequencing, Genome binding/occupancy profiling by high throughput sequencing|
|Number of samples:||20|
|Release date:||Dec 14 2018|
|Last update date:||Jan 23 2019|
|Dataset link||H3K27 tri-methyltransferases CLF and SWN redundantly buffer ABA-induced senescence|
Arabidopsis thaliana seedlings were grown on 1/2 Murashige and Skoog (MS) medium without sucrose (2% agar, PH 5.8) under LDs conditions. clf-50swn-1 is in the Ws background. For ABA treatment, 10-d old seedlings were transferred onto 1/2 MS medium containing 50 uM ABA or 100 uM ABA. After 4 days' treatment, the aerial parts were harvested, either frozen in liquid nitrogen for RNA isolation or directly vacuum-infiltrated with formaldehyde crosslinking solution for ChIP assay.