Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Sep 23 2009
Last update date: Jan 12 2017
Access: Public
Diseases: Bronchiolitis, Bronchiolitis, Viral
Dataset link Double-Stranded RNA-Activated Protein Kinase Regulates early innate immune responses during RSV Infection

Experimental Protocol


6-10 week old male WT (B6.129PF1/J) (The Jackson Labs, Bar Harbor, ME) and PKR-/- mice were used in all experiments. Mice were housed and maintained in specific pathogen-free conditions and used in accordance with the regulations of the National Institutes of Health. The human long strain of RSV-A2 (originally from Dr. B.S. Graham, NIH) was propagated in HEp-2 cells (American Type Culture Collection). Viral stocks were prepared by harvesting HEp-2 cells in fresh medium and three quick freeze thaw cycles followed by high speed centrifugation (10,000 X g) to remove cellular debris.

Repositories


GEO

GSE18170

BioProject

PRJNA119523

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Contact


NIEHS Microarray Core