|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Sep 23 2009|
|Last update date:||Jan 12 2017|
|Diseases:||Bronchiolitis, Bronchiolitis, Viral|
|Dataset link||Double-Stranded RNA-Activated Protein Kinase Regulates early innate immune responses during RSV Infection|
6-10 week old male WT (B6.129PF1/J) (The Jackson Labs, Bar Harbor, ME) and PKR-/- mice were used in all experiments. Mice were housed and maintained in specific pathogen-free conditions and used in accordance with the regulations of the National Institutes of Health. The human long strain of RSV-A2 (originally from Dr. B.S. Graham, NIH) was propagated in HEp-2 cells (American Type Culture Collection). Viral stocks were prepared by harvesting HEp-2 cells in fresh medium and three quick freeze thaw cycles followed by high speed centrifugation (10,000 X g) to remove cellular debris.
NIEHS Microarray Core