Computational protocol: Electric Field-Assisted Delivery of Photofrin to Human Breast Carcinoma Cells

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Protocol publication

[…] Cells were observed under MacroFluo Leica Z16 APO with PLANAPO 5.0×/0.50 LWD objective and 9.2 magnification (Leica, Germany). Exposure time was 10 ms (white light) and 1000 ms (fluorescent light). Cells were excited at 587 nm, fluorescence emission was read at 610 nm. Three images per condition were recorded. Images were analyzed with ImageJ software. The same brightness scale was set for all images in one experiment. Three-dimensional profiles of each single cell were obtained by the use of Interactive 3D Surface Plot Plugin to ImageJ software. Mean gray scale values were computed for each single cell in each fluorescent image separately and they were considered as equivalent of the fluorescent emission intensity of each single cell. Statistical analysis was performed with MATLAB 2011b (MathWorks). Statistical distribution of mean gray values was described with box plots. One box was created for each condition (each value of electric field intensity). In each box, the central mark is median, the edges of the box are the 25 and 75th percentiles, the whiskers extend to the most extreme data points not considered outliers, and outliers are plotted individually (marked with crosses). […]

Pipeline specifications

Software tools ImageJ, Interactive 3D Surface Plot
Application Microscopic phenotype analysis
Organisms Homo sapiens, Cricetulus griseus
Diseases Breast Neoplasms, Neoplasms
Chemicals Gentian Violet, Dihematoporphyrin Ether