Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 14
Release date: May 11 2010
Last update date: Feb 21 2017
Access: Public
Chemicals: Niacinamide, Thiamine
Dataset link Yeast expression data from conditions that inhibit sirtuins

Experimental Protocol


Duplicate cultures in rich YPD media were grown into log phase and then harvested. The deletion mutants npt1∆, bna1∆, tna1∆, and pnc1∆ were compared to a wild-type (WT) strain. Additionally, a WT strain grown in the presence of 5 mM NAM was compared to WT without NAM. Total RNA was isolated and used for microarray hybridizations onto Affymetrix Yeast 2.0 Gene arrrays.

Repositories


GEO

GSE18488

ArrayExpress

E-GEOD-18488

BioProject

PRJNA120233

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Contact


Jeffrey Smith
Mingguang Li
Veena Valsakumar