Computational protocol: Gold nanozyme-based paper chip for colorimetric detection of mercury ions

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Protocol publication

[…] Paper-based devices were fabricated using a commercially available wax printer (Color Cube 8870, Xerox, Norwalk, CT, USA) as previously reported. Hydrophobic patterns, which were designed using graphics software (Adobe Illustrator CS3), were printed on chromatography paper to define the fluidic path and control liquid transport. The paper was heated at 100 °C for 1 min in a dry oven to melt the patterned wax. The patterned paper (6.0 × 6.0 cm) was cut, and the backside of each piece of paper was fully covered by an adhesive PET film. To integrate AuNPs onto the paper chip, 1.5 μL of 20 nm AuNP solution (0.194 nM) was drop-dried on each test zone (with a diameter of 1.0 cm) at ambient temperature. [...] A 2 μL volume of sample was applied to each test zone of the paper chip. Then, 100 μL of 0.05% TMB solution containing 3.0% H2O2 was added to the reagent loading zone. This solution then flowed along the fluidic path until it reached the arch-shaped absorbent zones. The assay results were directly visible to the naked eye, amplifying over time. To quantify the results, after 10 min incubation, photographs were taken using a smartphone camera (LG-G5, 16.0 megapixels) with a shutter speed of 1/20 at ISO 50. Subsequently, colorimetric signal analysis was carried out using ImageJ software (National Institutes of Health, Bethesda, MD, USA). […]

Pipeline specifications

Software tools Adobe Illustrator, ImageJ
Applications Miscellaneous, Microscopic phenotype analysis
Chemicals Hydrogen Peroxide, Mercury