Computational protocol: Acetylcholinesterase in Biofouling Species: Characterization and Mode of Action of Cyanobacteria-Derived Antifouling Agents

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Protocol publication

[…] All data concerning ChEs activity characterization, in vivo anti-settlement tests and in vitro AChE activity were analysed using a one-way analysis of variance (ANOVA) followed by a multi-comparisons Dunnett test (p < 0.05). When ANOVA presuppositions were not met in the data, a non-parametric test, the Kruskal-Wallis on ranks, followed by the Dunn’s test, were used. ChEs activity kinetics for the different substrates was obtained according to Michaelis-Menten model []. A non-linear regression was used to fit data directly to the best hyperbola and the kinetic parameters, Km and Vm, for each substrate, were obtained through a hyperbolic regression analysis using the software STATISTICA 12. Km (μM) is the concentration of substrate at which half the active sites are filled, providing a measure of the substrate concentration required for significant catalysis to occur. Vm (nmol min−1 mg protein−1) represents the number of substrate molecules converted into product by the enzyme at full substrate saturation [].In the case of data from ChEs’ characterization using inhibitors, Dunnett’s test was used to compare each treatment with the control and to determine the values of the no observed effect concentration (NOEC) and the lowest observed effect concentration (LOEC) []. The software IBM SPSS Statistics 21 was used for statistical analysis. […]

Pipeline specifications

Software tools Statistica, SPSS
Application Miscellaneous