Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 12
Release date: Sep 13 2013
Last update date: Oct 10 2018
Access: Public
Diseases: Neoplasms, Nervous System Diseases
Dataset link Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery (RNA-Seq)

Experimental Protocol


Total RNA was extracted from control and Prmt5 depleted Neural Stem/Progenitors Cells (NPCs) and Mouse Embryonic Fibroblasts (MEFs). Prmt5 depleted cells were treated with 4-OHT 24 hours before splitting to induce PRMT5 knockout and final libraries were sequenced in triplicates on Illumina HiSeq 2000.

Repositories


GEO

GSE45284

ArrayExpress

E-GEOD-45284

ENA

SRP019782

BioProject

PRJNA193512

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Contact


Julius Müller