|Number of samples:||8|
|Release date:||Jan 23 2017|
|Last update date:||Jan 25 2017|
|Dataset link||Genome-wide transcriptional start site mapping and sRNA identification in the pathogen Leptospira interrogans|
To detect the transcripts expressed by L. interrogans and identify TSS, we collected cultures grown to the exponential phase at 30 and 37°C. Total RNA was extracted and subjected to rRNA depletion. Directional cDNA libraries were prepared from enriched fragmented mRNA. To allow selective enrichment of native 5′ ends of transcripts for the determination of transcription start sites (TSS), RNA samples were also treated with terminator 5‘-phosphatedependent exonuclease (TEX) followed by tobacco acid pyrophosphatase (TAP) treatment prior to cDNA library construction. The cDNA libraries of the different samples (30-TEX(-)TAP(-) / 30-TEX(-)TAP(+) / 30-TEX(+)TAP(+) / 30-RNAseq / 37-TEX(-)TAP(-) / 37-TEX(-)TAP(+) / 37-TEX(+)TAP(+) / 37-RNAseq) were sequenced on an Illumina HiSeq2500 instrument (Illumina).