Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 8
Release date: Jan 23 2017
Last update date: Jan 25 2017
Access: Public
Chemicals: Nucleotides
Dataset link Genome-wide transcriptional start site mapping and sRNA identification in the pathogen Leptospira interrogans

Experimental Protocol


To detect the transcripts expressed by L. interrogans and identify TSS, we collected cultures grown to the exponential phase at 30 and 37°C. Total RNA was extracted and subjected to rRNA depletion. Directional cDNA libraries were prepared from enriched fragmented mRNA. To allow selective enrichment of native 5′ ends of transcripts for the determination of transcription start sites (TSS), RNA samples were also treated with terminator 5‘-phosphatedependent exonuclease (TEX) followed by tobacco acid pyrophosphatase (TAP) treatment prior to cDNA library construction. The cDNA libraries of the different samples (30-TEX(-)TAP(-) / 30-TEX(-)TAP(+) / 30-TEX(+)TAP(+) / 30-RNAseq / 37-TEX(-)TAP(-) / 37-TEX(-)TAP(+) / 37-TEX(+)TAP(+) / 37-RNAseq) were sequenced on an Illumina HiSeq2500 instrument (Illumina).

Repositories


GEO

GSE92976

ENA

SRP095742

BioProject

PRJNA359198

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Contact


Anna ZHUKOVA