Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 12
Release date: Sep 5 2017
Last update date: Nov 20 2018
Access: Public
Chemicals: Ethanol
Computational protocol: BaseSpace, Bowtie2, TopHat, Partek Flow, Partek Pathway, Partek Genomics Suite, MetaboAnalyst, GEO, KEGG, KEGG Annotation
Dataset link Heterogeneity of p53 dependent genomic responses following ethanol exposure in a developmental mouse model of fetal alcohol spectrum disorder

Experimental Protocol


Somatosensory cortex was removed from wildtype (WT) and p53 knockout (KO) male mice on postnatal day 7, 8 hours after an IP injection of ethanol. Brains were flash frozen on dry ice, and RNA was harvested using Trizol reagent followed by RNeasy (Qiagen). Illumina Stranded RNA libraries were prepared using the RS-122-2101 Sample Prep Kit with 200-300 nanograms of total RNA for the starting material.

Repositories


GEO

GSE100217

ENA

SRP109699

BioProject

PRJNA391022

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Contact


Frank Middleton

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