Dataset features
Specifications
Application: | RNA-seq analysis |
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Number of samples: | 12 |
Release date: | Jul 20 2018 |
Last update date: | Nov 27 2018 |
Access: | Public |
Dataset link | Transcriptome analysis using RNA sequencing conducted for wild type (Col-0) and ahl10-1 mutant under control conditions and after 96 h at moderate low water potential (-0.7 MPa) |
Experimental Protocol
Wild type (Col-0) and ahl10-1 Arabidopsis thaliana seedlings were germinated on agar plates and after seven days of growth, seedlings were transferred either to fresh plates of control media or to moderate severity low water potential (-0.7 MPa) imposed using agar plates of the control media infiltrated with PEG-8000. Samples were collected 96 h after transfer (Control and stress treatments). Three independent biological experiments and mRNA extractions were performed for each genotype/treatment. After quality check and quantitation, the samples were used for sequencing
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Verslues Paul