Computational protocol: Trypanin, a Component of the Flagellar Dynein Regulatory Complex, Is Essential in Bloodstream Form African Trypanosomes

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Protocol publication

[…] Protein sequence alignments were performed using the Align X program in Vector NTI Advance (Informax, Frederick, Maryland, United States), which utilizes the Clustal W algorithm []. Phylogenetic tree assembly is based on the sequence distance method and utilizes the Neighbor Joining (NJ) algorithm []. Note that kinetoplastids contain a second trypanin-related protein [], which was excluded from this phylogenetic analysis for simplicity. […]

Pipeline specifications

Software tools Vector NTI Advance, Clustal W
Applications Phylogenetics, Protein sequence analysis
Organisms Trypanosoma brucei