Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jun 15 2010
Last update date: Oct 29 2018
Access: Public
Diseases: Soft Tissue Infections
Dataset link EWS-FLI1 reactivates a neural crest stem cell program in human neural crest-derived mesenchymal stem cells

Experimental Protocol


3 replicate samples for 4 different stem cell populations were analyzed by HuEx arrays. The 4 sample types were adult human bone marrow-derived mesenchymal stem cells, human embryonic stem cell-derived nueral crest stem cells (hNCSC), control vector-transduced hNCSC-derived mesenchymal stem cells (NC-MSC), and EWS-FLI1-transduced hNCSC-derived mesenchymal stem cells (NC-MSC EF). Control and EWS-FLI1 transduced NC-MSC were isolated 5 days after lentiviral transduction. Transcript level expression data was compared among the different populations to determine differences and similarities between NCSC, BM-MSC and NC-MSC with/without EWS-FLI1 expression. These data were used to identify EWS-FLI1 targets in NC-MSC and to characterize the genetic changes that occur in NCSC as they generate NC-MSC progeny.

Repositories


GEO

GSE21511

ArrayExpress

E-GEOD-21511

BioProject

PRJNA125985

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Contact


Elizabeth Lawlor
Elizabeth R Lawlor

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