|Number of samples:||10|
|Release date:||Jan 15 2008|
|Last update date:||Mar 19 2012|
|Dataset link||Maintenance of Long-Range DNA Interactions after Inhibition of Ongoing RNA Polymerase II Transcription.|
4C experiments were performed in duplo, dye swap was included. Hybridisation was performed on dedicated micro-arrays. Probes (60-mers) were selected from the sequences 100 bp up –and downstream of HindIII sites. To prevent cross-hybridization, probes that had any similarity with highly abundant repeats (RepBase 10.09) were removed from the probeset. In addition, probes that gave more than two BLAST hits in the genome were also removed from the probeset. Sequence alignments were performed using MegaBLAST using the standard settings. A hit was defined as an alignment of 30 nt or longer.
Wouter de Laat
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