|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Jan 15 2007|
|Last update date:||Jan 17 2013|
|Diseases:||Lung Neoplasms, Neoplasms|
|Dataset link||miRNA let-7a-3 overexpression in lung cancer cell line A549|
Gene expression analysis comprised 4 microarray hybridizations: 4 let-7a-3 transfected cell culture replicates and 4 parental cell culture replicates (controls). Each let-7a-3 expressing replicate was hybridized against a control replicate of the same cell line including a dye swap design. Rawdata gpr files were processed using software ArrayMagic (Buness et al. 2005) including VSN normalization available on Bioconductor open source software platform. Differentially expressed genes between let-7a-3 transfected and control cell lines were analyzed by LIMMA (Wettenhall and Smyth, 2004) considering biological replicates and dye swap design.