Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Jan 15 2007
Last update date: Jan 17 2013
Access: Public
Diseases: Lung Neoplasms, Neoplasms
Dataset link miRNA let-7a-3 overexpression in lung cancer cell line A549

Experimental Protocol


Gene expression analysis comprised 4 microarray hybridizations: 4 let-7a-3 transfected cell culture replicates and 4 parental cell culture replicates (controls). Each let-7a-3 expressing replicate was hybridized against a control replicate of the same cell line including a dye swap design. Rawdata gpr files were processed using software ArrayMagic (Buness et al. 2005) including VSN normalization available on Bioconductor open source software platform. Differentially expressed genes between let-7a-3 transfected and control cell lines were analyzed by LIMMA (Wettenhall and Smyth, 2004) considering biological replicates and dye swap design.

Repositories


GEO

GSE6474

BioProject

PRJNA98711

Download