Gene expression profiling of murine plasmocytes isolated from the spleen of IL-10eGFP mice infected with Salmonella

Gene expression profiles of IL-10eGFP+CD138hi and IL-10eGFP-CD138hi cells. The experiment contains 2 groups. One group corresponds to IL-10eGFP+CD138hi cells, and the other group corresponds to IL-10eGFP-CD138hi cells. These cells were isolated from the spleen of IL-10eGFP mice (C57BL/6 genetic background) on day 1 after intravenous challenge with Salmonella typhimurium (strain SL7207, 10e7 CFU). The experiment was done in triplicates so there were three samples for each group. After total RNA extraction, reverse transcription, cDNA extraction, and in vitro transcription the biotinylated cRNA was fragmented, and hybridized on Affymetrix mouse genome 430 2.0 whole genome arrays. To obtain genes differentially expressed when comparing expression profiles of IL-10eGFP+CD138hi and IL-10eGFP-CD138hi cells group-wise comparisons were performed.