Computational protocol: Anti-proliferative and pro-apoptotic effects induced by simultaneous inactivation of HER1 and HER2 through endogenous polyclonal antibodies

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Protocol publication

[…] Cells were stained with specific antibodies during 20 min. Anti-EGFR PAb were diluted 1:200-1:3200. MAbs nimotuzumab or trastuzumab were used at 10μg/mL and 1μg/mL, respectively. Staining was followed by incubation with FITC-linked goat anti-mouse IgG or FITC-linked goat anti-human IgG for murine PAbs and human MAbs, respectively (Sigma). All analyses were performed using a Gallios flow cytometer (Beckton Dickinson, San Jose, CA, USA) and data were processed using Kaluza 1.2 software apart from Cell cycle analyses in which FlowJo 5.6 software was used. […]

Pipeline specifications

Software tools Kaluza, FlowJo
Application Flow cytometry
Organisms Homo sapiens
Chemicals Tyrosine