Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 36
Release date: Nov 11 2014
Last update date: Aug 14 2018
Access: Public
Dataset link Detection of Pol IV-dependent transcripts at the genomic scale in Arabidopsis reveals features and regulation of siRNA biogenesis

Experimental Protocol


To detect siRNA precursors transcribed by RNA polymerase IV, the genome wide profiling of RNA were carried out at dcl234 and dcl234 nrpd1. Different types of RNA (including Total RNA, polyA+ RNA, polyA- RNA, double stranded RNA) libraries were built to detect different transcripts. RDR2 is a RNA-dependent RNA polymerase in Pol IV complex, so the RNA-seq libraries with the mutation of RDR2 were also built. In addition, smRNA libraries with mutations blocking siRNA biogenesis were also built

Repositories


GEO

GSE57215

ArrayExpress

E-GEOD-57215

ENA

SRP041593

BioProject

PRJNA245920

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