Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 147
Release date: Mar 2 2018
Last update date: Nov 27 2018
Access: Public
Diseases: Neoplasms, Prostatic Neoplasms
Chemicals: Androgens
Dataset link Single-cell RNA-seq reveals a subpopulation of prostate cancer cells with enhanced cell cycle-related transcription and attenuated androgen response

Experimental Protocol


For each of 3 treatment groups, forty eight LNCaP single cells and 1 representative bulk cell RNA sample (1ng) were collected for SMART-seq2 amplification and later single-cell RNA-seq (total 144 single cells and 3 bulk cell samples). All of the treatment groups were harvested from after synchronizing the cells at the G1/S phase with a double thymidine block and androgen depriving the cells for ~24 hours. Treatment groups 2 and 3 were cultured in the absence and presence of androgen (1 nM R1881) for 12 hours, respectively. Treatment group 1 was a baseline comparison treatment group and was collected right after cell synchronization and androgen deprivation (considered 0 hour).

Repositories


GEO

GSE99795

ENA

SRP108807

BioProject

PRJNA389624

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Contact


Tim Huang