Dataset features

Specifications


Application: RNA-seq analysis, BS-seq analysis
Number of samples: 64
Release date: Oct 25 2017
Last update date: Nov 26 2018
Access: Public
Taxon: Mus musculus
Dataset link Cis-regulatory landscape of four cell types of the retina

Experimental Protocol


We generated genome-wide DNA methylation maps of four cell types isolated from mouse retina using FACS-sorting isolated from transgenic animals carrying markers for cones, rods, HCs and SBACs. We used this information to design libraries of candidate CRE (based on hypomethylation-LMRs) that were cloned into a parallelized transcriptional reporter construct (PRA) and packaged in adeno-associated viruses (AAVs) for batch delivery into mouse eyes. We measured the activity of hundreds of CREs in the four individual cell types. Samples originate from starburst amacrine cells, cones, rods, horizontal cells and whole retina.

Repositories


GEO

GSE84589

ENA

SRP078942

BioProject

PRJNA330531

Download


Dataset Statistics

info

Citations per year

Dataset publication