|Application:||RNA-seq analysis, BS-seq analysis|
|Number of samples:||64|
|Release date:||Oct 25 2017|
|Last update date:||Nov 26 2018|
|Dataset link||Cis-regulatory landscape of four cell types of the retina|
We generated genome-wide DNA methylation maps of four cell types isolated from mouse retina using FACS-sorting isolated from transgenic animals carrying markers for cones, rods, HCs and SBACs. We used this information to design libraries of candidate CRE (based on hypomethylation-LMRs) that were cloned into a parallelized transcriptional reporter construct (PRA) and packaged in adeno-associated viruses (AAVs) for batch delivery into mouse eyes. We measured the activity of hundreds of CREs in the four individual cell types. Samples originate from starburst amacrine cells, cones, rods, horizontal cells and whole retina.
Citations per year
Nucleic Acids Res