Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 11
Release date: Dec 31 2011
Last update date: May 15 2017
Access: Public
Chemicals: Doxorubicin
Dataset link RIP-chip of HuR in MCF7 cells exposed to doxorubicin

Experimental Protocol


We subjected MCF7 cells to starvation for 24h and then we added doxorubicin at final concentration of 10 uM, profiling before and after 4 hours of treatment in biological quadruplicate (only on cytoplasmic mRNAs, as HuR was found in the cytoplasm). Differentially expressed genes, altered during the treatment, were identified. Data derived from HuR RIP-Chip and IgG RIP-Chip (in biological quadruplicate) allowed the identification of specific mRNAs bound to HuR. The comparison between HuR RIP-Chip and cytoplasmic extracts from doxorubicin treated cells (in biological triplicate) identified those genes that were more strictly bound to HuR independently from their expression levels.

Repositories


GEO

GSE33055

ArrayExpress

E-GEOD-33055

BioProject

PRJNA149461

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Contact


Toma Tebaldi

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