Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 10
Release date: Aug 26 2017
Last update date: Nov 28 2018
Access: Public
Chemicals: Puromycin
Dataset link Human Adult Sorted Live Cell Erythroblasts transduced with Sigma shRNA Clone TRCN0000005418 targeting RIOK3 with puromycin selection RNAseq

Experimental Protocol


Human CD34+ cells from health donor transduced with lentivirus expressing Sigma shRNA Clone TRCN0000005418 to knockdown RIOK3 gene expression in serum free culture. RNA extracted from sorted live cell gate on Day 14 using Qiagen miRNeasy Mini Kit. 1ug of RNA was used for library construction and Ribo depletion with globin subtraction. We would like to thank Gerard Bouffard and the National Human Genome Research Institute for their expertise and assistance with RNA-seq.

Repositories


GEO

GSE103122

ENA

SRP116196

BioProject

PRJNA400217

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Contact


Jeffery Miller