Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 50
Release date: Oct 16 2014
Last update date: Nov 17 2017
Access: Public
Taxon: Drosophila melanogaster
Dataset link Establishment of regions of genomic activity during the Drosophila maternal-to-zygotic transition

Experimental Protocol


We performed genome-wide mapping of histone H3 and 9 types of histone modifications, including H4K5ac, H4K8ac, H3K4me1, H3K4me3, H3K27me3, H3K36me3, H3K9ac, H3K18ac, and H3K27ac by ChIP-seq, in hand-sorted wild-type Drosophila melanogaster embryos at 4 different development time points corresponding to mitotic cycle 7-9, 11-13, 14a-b, and 14c-d, respectively. We also carried out ChIP-seq experiments in zelda mutant embryos after showing that the deposition of histone marks in early embryos strongly correlated with the binding of Zelda in wild-type embryos.

Repositories


GEO

GSE58935

ArrayExpress

E-GEOD-58935

ENA

SRP044032

BioProject

PRJNA254188

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Contact


Michael Eisen
Michael B Eisen

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