Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Mar 6 2015
Last update date: May 4 2018
Access: Public
Diseases: Adrenal Gland Neoplasms, Breast Neoplasms, Neoplasms
Chemicals: Tyrosine
Dataset link Shp2 Signaling Suppresses Senescence in PyMT-induced Mammary Gland Cancer in Mice [Mouse430A_2 array]

Experimental Protocol


For Shp2 ablation, primary mammary tumor cells from MMTV-PyMT;Shp2fl/fl mice were transduced with retroviruses expressing control GFP or CreERT2-GFP, and GFP-positive cells were purified by FACS and cultured as mammospheres for 1 week. Mammospheres were then treated with 50 nM 4-OHT for 2 hours, cultured for another 5 days, and subjected to RNA isolation. For pharmacological inhibition, primary mammary tumor cells from the same mice were cultured as mammospheres for 10 days and then treated with the Shp2 inhibitor GS493 for another 2 days. Mammospheres were then subjected to RNA isolation. Each group contains 3 replicates.

Repositories


GEO

GSE50516

ArrayExpress

E-GEOD-50516

BioProject

PRJNA217933

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