Computational protocol: Invasive mutualisms between a plant pathogen and insect vectors in the Middle East and Brazil

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Protocol publication

[…] We selected 10 citrus orchards known to have highest incidences of WBDL in Oman []. Ten leaf samples each from symptomatic and asymptomatic A. conyzoides and P. maderaspatensis were collected from a total of 133 plants (in some instances not all four combinations were to be found). Observed symptoms were similar to WBDL symptoms: small shoots, leaf and shoot chlorosis and shortening of internodes (electronic supplementary material, figure S2). DNA extraction, PCR analysis and sequencing were performed as described above using nested PCR P1/P7-R16F2n/R16R2 (electronic supplementary material, table S3). Sequence comparisons were made using megaBLAST [] against reference sequences (NCBI GenBank accession number: U15442). Sequences were aligned with ClustalW2 and phylogenetic trees were constructed using MEGA5 v. 5.05 (PA, USA), with neighbour-joining method (bootstrap replication n = 500; []. Acholeplasma palmae was selected as the out-group. […]

Pipeline specifications

Software tools BLASTN, Clustal W, MEGA
Application Phylogenetics
Organisms Citrus aurantiifolia, Diaphorina citri