Computational protocol: Sarcosine Up-Regulates Expression of Genes Involved in Cell Cycle Progression of Metastatic Models of Prostate Cancer

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Protocol publication

[…] For the statistical evaluation of the results, the mean was taken as the measurement of the main tendency, while standard deviation was taken as the dispersion measurement. Differences between groups were analyzed using paired t-test and ANOVA. Unless noted otherwise, the threshold for significance was p < 0.05. For analyses Software Statistica 12 (StatSoft, Tulsa, OK, USA) was employed. The annotation analyses were performed using the GoMiner (http://discover.nci.nih.gov/gominer/index.jsp), interactome network was constructed using the STRING software (http://string-db.org/) and the PCa metabolic pathway was visualized using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database (http://www.genome.jp/kegg/), which provides gold standard sets of molecular pathways. The involvement of genes involved in a cell cycle was carried out using the Reactome (www.reactome.org). […]

Pipeline specifications

Software tools Statistica, GoMiner
Databases Reactome KEGG KEGG PATHWAY
Application Miscellaneous
Organisms Mus musculus
Diseases Neoplasms, Prostatic Neoplasms
Chemicals Amino Acids, Glycine, Sarcosine