|Application:||Gene expression microarray analysis|
|Number of samples:||10|
|Release date:||Aug 14 2012|
|Last update date:||Nov 14 2012|
|Computational protocol:||DBTBS, Cyber-T, Genome2D|
|Dataset link||High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis|
Bacillus subtilis strain MP902 [strain MP901 (Ptet-ccpA, KmR) carrying pWH119 (Pxyl-tetR, EmR)] was grown in presence of three different concentrations of Ptet inducer, anhydrotetracycline (ATc), leading to three levels of ccpA expression induction. The control culture was grown in absence of ATc. The total RNA for transcriptome analyses was isolated at OD600 = 0.8 from 16 ml culture. Three independent cultures of each strain (target strains and controls) were used, and cells were sampled for microarray experiment.
DOI: 10.1186/1471-2164-13-401call_split See protocol