Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 10
Release date: Aug 14 2012
Last update date: Nov 14 2012
Access: Public
Chemicals: Carbon
Computational protocol: DBTBS, Cyber-T, Genome2D
Dataset link High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis

Experimental Protocol


Bacillus subtilis strain MP902 [strain MP901 (Ptet-ccpA, KmR) carrying pWH119 (Pxyl-tetR, EmR)] was grown in presence of three different concentrations of Ptet inducer, anhydrotetracycline (ATc), leading to three levels of ccpA expression induction. The control culture was grown in absence of ATc. The total RNA for transcriptome analyses was isolated at OD600 = 0.8 from 16 ml culture. Three independent cultures of each strain (target strains and controls) were used, and cells were sampled for microarray experiment.

Repositories


GEO

GSE35154

ArrayExpress

E-GEOD-35154

BioProject

PRJNA150777

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