|Application:||Gene expression microarray analysis|
|Number of samples:||13|
|Release date:||Jan 31 2008|
|Last update date:||Aug 28 2018|
|Dataset link||Gene Expression in MCF10A cells through Differentiation on Transwells|
Cells are grown in monolayer to 90-95% confluency, trypsinized and counted for seeding onto permeable supports (Transwell®, 0.4 µm pores, polyester) in normal growth medium. MCF10A cells are seeded on 12-well Transwells® (Corning) at 105 cells/cm2. Both chambers of media were changed strictly on a 24-hour schedule. Transepithelial electrical resistance (TEER) is measured daily with Epithelial Volt-Ohm Meter (EVOM; World Precision Instruments), prior to media change. Total RNA was isolated at the indicated TEER listed above. Canonical monolayer MCF10A cells served as a control comparison. Each time point was performed on triplicate arrays except for Plateau (n=4).
Nelson D. Horseman