Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 13
Release date: Jan 31 2008
Last update date: Aug 28 2018
Access: Public
Taxon: Homo sapiens
Dataset link Gene Expression in MCF10A cells through Differentiation on Transwells

Experimental Protocol


Cells are grown in monolayer to 90-95% confluency, trypsinized and counted for seeding onto permeable supports (Transwell®, 0.4 µm pores, polyester) in normal growth medium. MCF10A cells are seeded on 12-well Transwells® (Corning) at 105 cells/cm2. Both chambers of media were changed strictly on a 24-hour schedule. Transepithelial electrical resistance (TEER) is measured daily with Epithelial Volt-Ohm Meter (EVOM; World Precision Instruments), prior to media change. Total RNA was isolated at the indicated TEER listed above. Canonical monolayer MCF10A cells served as a control comparison. Each time point was performed on triplicate arrays except for Plateau (n=4).

Repositories


GEO

GSE10070

ArrayExpress

E-GEOD-10070

BioProject

PRJNA108175

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Contact


Nelson Horseman
Nelson D. Horseman