Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 32
Release date: Feb 10 2006
Last update date: Feb 18 2018
Access: Public
Chemicals: Glucose, Lactose
Dataset link Metabolic regulation in the lactating mammary gland:A lipid synthesizing machine

Experimental Protocol


FVB control time-series mice were on normal lab chow diets and mammary tissue was taken at Pregnant days 12 and 17, plus lactation days 1, 2, and 9 in those animals. the time-points were taken as quadruplicates for each developmental stage. for the dietary component, triplicate FVB dams were fed normal lab chow diets until lactation day 5, when they were converted to diets containing either 8% or 40% Kcal from soy oils (see supplementary table to publication for further detail) for 5 days. At lactation day 10, mammary and liver tissues were taken from these animals and preparred for microarray analysis. All tissues were stabilized for RNA content in RNALater and were then isolated and cleaned up following manufacturer's instructions (Qiagen).

Repositories


GEO

GSE4222

ArrayExpress

E-GEOD-4222

BioProject

PRJNA94865

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Contact


Michael Rudolph
Michael C. Rudolph