Computational protocol: Aromatic amino acids in the cellulose binding domain of Penicillium crustosum endoglucanase EGL1 differentially contribute to the cellulose affinity of the enzyme

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Protocol publication

[…] A homology model of the three-dimensional structure of the catalytic domain (glucanase) was generated using SWISS-MODEL [] with the family 1 carbohydrate-binding module from Trichoderma reesei Cel7A (PDB code: 2MWK) as the template, which is 63% identical to the CBD domain of P. crustosum endoglucanase EGL1 []. The model quality was assessed using the MolProbity server [] with a MolProbity score of 2.77. The figures were prepared with PyMOL (Schrödinger, Cambridge, MA). […]

Pipeline specifications

Software tools SWISS-MODEL, MolProbity, PyMOL
Application Protein structure analysis
Chemicals Amino Acids, Glucose, Glycine