Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 12
Release date: May 18 2014
Last update date: Aug 10 2018
Access: Public
Taxon: Homo sapiens
Dataset link Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

Experimental Protocol


1.sgRNA1-6 binding sites were identified with ChipSeq by using HA antibody (there are 2 replicates for sgRNA1-3, one sample for sgRNA4-6,one control without sgRNA) 2.PCR products which amplifies " off-target genomic sites" were deep sequenced in the presence of WT Cas9+sgRNA or WT Cas9 alone( unique adaptor was used for each sgRNA and mixed for multiplex run)

Repositories


GEO

GSE55887

ArrayExpress

E-GEOD-55887

ENA

SRP040086

BioProject

PRJNA241244

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Contact


Mazhar Adli