|Application:||Gene expression microarray analysis|
|Number of samples:||31|
|Release date:||Aug 24 2013|
|Last update date:||Aug 26 2013|
|Chemicals:||Ammonia, Nitrates, Nitrogen|
|Dataset link||Hydrodynamic conditions shape ammonia-oxidizer communities in the central Baltic Sea water column|
Two color array (Cy3 and Cy5): the universal standard 20-mer oligo is printed to the slide with a 70-mer oligo (an archetype). Environmental DNA sequences (fluoresced with Cy3) within 15% of the 70-mer conjugated to a 20-mer oligo (fluoresced with Cy5) complementary to the universal standard will bind to the oligo probes on the array. Signal is the ratio of Cy3 to Cy5. Three replicate probes were printed for each archetype. Two replicate arrays were run on duplicate targets.
Bess B Ward