Computational protocol: Lipoprotein biosynthesis as a target for anti-Wolbachia treatment of filarial nematodes

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Protocol publication

[…] The LspA protein sequences from different Wolbachia strains and other reference species (shown in Figure ) were retrieved from NCBI Genbank database [] via protein-protein BLAST similarity searches and were aligned using CLUSTALX 1.83 []. The sequences in the alignment were further analyzed by Genedoc 2.6 [] for calculating percentage of amino acid identity/similarity, TMHMM 2.0 [] for prediction of transmembrane domains, and SignalP 3.0 [] for predicting signal peptide. […]

Pipeline specifications

Software tools DELTA-BLAST, Clustal W, TMHMM, SignalP
Applications Membrane protein analysis, Amino acid sequence alignment
Organisms Caenorhabditis elegans, Brugia malayi, Escherichia coli, Aedes albopictus
Diseases Elephantiasis, Filarial, Onchocerciasis