Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Mar 30 2018
Last update date: May 2 2018
Access: Public
Dataset link C. elegans FBF iCLIP from spermatogenic and oogenic germlines

Experimental Protocol


We generated FBF-1 and FBF-2 iCLIP datasets from animals with somatic tissues of the same gender but germline tissues of opposite gender. For each FBF, we used an N-terminally FLAG-tagged single copy fbf transgene in a strain lacking the endogenous gene (e.g. tagged FLAG::FBF-1 in an fbf-1 null mutant ). Adult hermaphrodites with a sexually transformed germline that are strictly spermatogenic were obtained using a temperature sensitive gain of function mutation in the fem-3 gene (Barton and Kimble 1987). These fem-3 (ts, gf) mutants are spermatogenic at restrictive temperature (25°C). We therefore introduced FLAG-tagged FBF-1 or FBF-2 into the fem-3(ts,gf) mutant, again removing the corresponding endogenous fbf gene in each strain. We then performed FBF iCLIP from adults that were either oogenic or spermatogenic, both raised at 25 °C. For each strain (each FBF, each germline gender), we processed three biological replicates. Three negative control datasets were produced for each germline gender in parallel by omitting the FLAG antibody.

Repositories


GEO

GSE83695

ENA

SRP077050

BioProject

PRJNA326738

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