Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Dec 31 2015
Last update date: Jan 1 2016
Access: Public
Taxon: Homo sapiens
Dataset link Evaluation of ribonucleic acid amplification protocols for human oocyte transcriptome analysis

Experimental Protocol


Design: Five commercially available protocols were investigated for their efficiency to amplify messenger RNA (mRNA) from 54 single human oocytes. Protocols resulting in sufficient yields were further validated using microarray technology. For the validation, mRNA was isolated from 25 human oocytes. To eliminate biological variation, RNA from 13 human oocytes was pooled together and split into 12 identical samples for further mRNA amplification. From 12 oocytes, mRNA was individually isolated.

Repositories


GEO

GSE48141

ArrayExpress

E-GEOD-48141

BioProject

PRJNA209022

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Contact


Martijs Jonker
Paul Wackers