|Application:||Gene expression microarray analysis|
|Number of samples:||16|
|Release date:||Dec 31 2015|
|Last update date:||Jan 1 2016|
|Dataset link||Evaluation of ribonucleic acid amplification protocols for human oocyte transcriptome analysis|
Design: Five commercially available protocols were investigated for their efficiency to amplify messenger RNA (mRNA) from 54 single human oocytes. Protocols resulting in sufficient yields were further validated using microarray technology. For the validation, mRNA was isolated from 25 human oocytes. To eliminate biological variation, RNA from 13 human oocytes was pooled together and split into 12 identical samples for further mRNA amplification. From 12 oocytes, mRNA was individually isolated.