Computational protocol: KELCH F-BOX protein positively influences Arabidopsis seed germination by targeting PHYTOCHROME-INTERACTING FACTOR1

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Protocol publication

[…] Primers for real-time qPCR were made to ACTIN2, PIF1, and CTG10 (Table S1) cDNA using IDT PrimerQuest (Integrated DNA Technologies, Inc.) and the Primer3 program (, ) and were analyzed using NetPrimer (PREMIER Biosoft; www.premierbiosoft.com/netprimer/). A Bio-Rad Laboratories iCycler permitted measurement of SYBR Green fluorescence to determine abundance of the PIF1 and CTG10 transcripts relative to ACTIN2. […]

Pipeline specifications

Software tools PrimerQuest, Primer3, NetPrimer
Application qPCR
Organisms Arabidopsis thaliana, Caenorhabditis elegans