Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 15
Release date: Apr 8 2014
Last update date: Oct 11 2016
Access: Public
Genes: PPARGC1A, PRKAA1, ATIC
Taxon: Homo sapiens
Dataset link Inadequate mito-biogenesis in primary dermal fibroblasts from old humans is associated with impairment of PGC1A-independent stimulation.

Experimental Protocol


Donors: 15 human female donors included in the study were aged 20, 21, 23, 26, 26, 40, 41, 42, 43, 49, 60, 62, 63, 64 and 67 years, thus covering the age spectrum 20 – 67 years and providing five biological replicates for each of the age groups “young” (20-30 years), “middle” (40-50 years) and “old” (60-70 years). Human dermal fibroblasts were isolated from skin specimen removed in the course of cosmetic surgery from the bottom side of female breast. Isolation and primary culture of the cells followed published procedures (Tigges and others 2013). Cells were not expanded beyond 12 population doublings, while replicative cell cycle arrest was determined to not occur before 40 population doublings.

Repositories


GEO

GSE55118

ArrayExpress

E-GEOD-55118

BioProject

PRJNA238579

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Contact


Hauke Busch