Computational protocol: Variation in the wheat AP2 homoeologs, the genes underlying lodicule development

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Protocol publication

[…] Multiple sequence alignments at both the nucleotide and predicted polypeptide level were performed using DNAMAN v6.0 software (Lynnon Biosoft, Quebec, Canada). Phylogenetic trees were constructed based on the neighbor-joining method, using MEGA v5 software (). Bootstrap analysis was based on 1,000 replicates. […]

Pipeline specifications

Software tools DNAMAN, MEGA-V
Applications Phylogenetics, GWAS
Organisms Triticum aestivum, Hordeum vulgare