Computational protocol: A new horsehair worm, Chordodes formosanus sp. n. (Nematomorpha, Gordiida) from Hierodula mantids of Taiwan and Japan with redescription of a closely related species, Chordodes japonensis

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Protocol publication

[…] Genomic DNA was extracted from fragments of horsehair worms and whole larvae using an ALS Tissue Genomic DNA Extraction Kit (Kaohsiung, Taiwan). A partial COI sequence was amplified by a polymerase chain reaction (PCR) with a set of universal primers (LCO1490 and HC02198) (). The PCR was initiated at 95°C for 5 min, followed by 35 cycles at 95°C for 1 min, 40°C for 1 min, and 72°C for 1 min, with a final extension at 72°C for 7 min. The PCR products were obtained by electrophoresis in 1.5% agarose gels and sequenced.For the phylogenetic analysis, the COI sequence of Paragordius sp. (GenBank no. AY428843) was used as an outgroup. The 528 nucleotide base pairs of high quality were aligned using CLUSTALX 2.0.10 (). Pairwise genetic distances were calculated, and a phylogenic tree was reconstructed by the Neighbor-joining (NJ) method based on the Kimura 2-parameter model using MEGA 4.0.2 (). The support for the topology of the NJ tree was estimated by bootstrapping using 1000 replicates. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics