Computational protocol: Pleiotropic Associations of RARRES2 Gene Variants and Circulating Chemerin Levels: Potential Roles of Chemerin Involved in the Metabolic and Inflammation-Related Diseases

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Protocol publication

[…] Genomic DNA was extracted as previously reported []. Oligonucleotide primers were generated to amplify genomic DNA fragments containing single-nucleotide polymorphisms (SNPs), as reported in the National Center for Biotechnology Information SNP database (http://www.ncbi.nlm.nih.gov/SNP). On the basis of the HapMap database, we analyzed six SNPs with selected tagSNPs by running the tagger program implemented in SNPinfo Web Server and with the coverage of the RARRES2 region (encoding chemerin; linkage disequilibrium [LD] threshold = 0.8 and minor allele frequency ≥ 0.2). Genotyping for the studied polymorphisms was performed using TaqMan SNP genotyping assays obtained from Applied Biosystems (ABI, Foster City, CA, USA). […]

Pipeline specifications

Software tools Tagger, SNPinfo
Application GWAS
Diseases Metabolic Diseases
Chemicals Cholesterol, Tretinoin