|Number of samples:||81|
|Release date:||Apr 6 2018|
|Last update date:||Nov 28 2018|
|Diseases:||Autoimmune Diseases, Neoplasms|
|Dataset link||Time-course RNA-sequencing of human induced regulatory T cell (iTreg) differentiation|
We performed a time-course experiment including six time points and four iTreg differentiation conditions. We included also a control time series and unstimulated nTregs. Three biological replicates were included. The total sample count is 81. In detail, human naïve CD4+ T cells were magnetically negatively isolated from peripheral blood. Cells were stimulated in serum-free medium with anti-CD3/anti-CD28 antibodies plus IL-2, and samples were taken at 2h, 6h, 24h, 48h and 6d of stimulation. Mock stimulation control cells (sample group G02) received no further compounds, whereas induced regulatory T cells (iTregs) were either differentiated under addition of TGF-b (sample group G03), TGF-b + retinoic acid (sample group G04), TGF-b + retinoic acid + rapamycin (sample group G05) or TGF-b + butyrate (sample group G06). As control, naïve CD4+ T cells were left unstimulated (0h; sample group G01). Ex vivo isolated CD25-high cells were included as positive control for the Treg signature (“nTreg”; sample group G07). Tregs were defined by expression of FOXP3, the “master” transcription factor of Tregs.
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