Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 81
Release date: Apr 6 2018
Last update date: Nov 28 2018
Access: Public
Diseases: Autoimmune Diseases, Neoplasms
Dataset link Time-course RNA-sequencing of human induced regulatory T cell (iTreg) differentiation

Experimental Protocol


We performed a time-course experiment including six time points and four iTreg differentiation conditions. We included also a control time series and unstimulated nTregs. Three biological replicates were included. The total sample count is 81. In detail, human naïve CD4+ T cells were magnetically negatively isolated from peripheral blood. Cells were stimulated in serum-free medium with anti-CD3/anti-CD28 antibodies plus IL-2, and samples were taken at 2h, 6h, 24h, 48h and 6d of stimulation. Mock stimulation control cells (sample group G02) received no further compounds, whereas induced regulatory T cells (iTregs) were either differentiated under addition of TGF-b (sample group G03), TGF-b + retinoic acid (sample group G04), TGF-b + retinoic acid + rapamycin (sample group G05) or TGF-b + butyrate (sample group G06). As control, naïve CD4+ T cells were left unstimulated (0h; sample group G01). Ex vivo isolated CD25-high cells were included as positive control for the Treg signature (“nTreg”; sample group G07). Tregs were defined by expression of FOXP3, the “master” transcription factor of Tregs.

Repositories


GEO

GSE94396

ENA

SRP098715

BioProject

PRJNA369563

Download


Contact


Francesco Marabita

Dataset Statistics

info

Citations per year

Dataset publication