Dataset features

Specifications


Application: Gene expression microarray analysis, ChIP-seq analysis
Number of samples: 22
Release date: Nov 28 2011
Last update date: Aug 10 2018
Access: Public
Diseases: Tumor Lysis Syndrome, Lymphoma, B-Cell
Computational protocol: Bowtie, HOMER, DESeq, ChIPpeakAnno, liftoveR, CoreSearch, limma, DAVID, ElDorado
Dataset link Deep sequencing of MYC DNA-binding sites in Burkitt's lymphoma

Experimental Protocol


[ChIP-Seq] Analysis of MYC DNA binding sites by ChiP-Seq in 5 Burkitt´s lymphoma cell lines (Raji, Ramos, Blue1, BL41, CA46) [mRNA expression profiling] siRNA-mediated knock-down of MYC was done employing the BL cell lines Raji, BL41 and Blue1 in order to detect MYC-driven gene expression changes. For this purpose, the cells were Amaxa-transfected using MYC smart pool siRNA and control siRNA (Thermo Scientific/Dharmacon, Erembodegem, Belgium), respectively.

Repositories


GEO

GSE30726

ArrayExpress

E-GEOD-30726

ENA

SRP008015

BioProject

PRJNA144391

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Contact


Volkhard Seitz

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