Computational protocol: Analysis of the correlation between adiponectin gene polymorphism and metabolic syndrome incidence and its relationship with the degree of atherosclerosis in patients

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Protocol publication

[…] After separating the target protein using 12% SDS gel, a cross-flow transferring membrane using 350 mA current was performed for 4 h, and the sample was sealed using 10% BSA for 1 h. The primary antibody dilution was performed in accordance with the antibody specification. After 4°C overnight, the membrane was washed three times with TBST buffer for 20 min. Then, it was diluted in accordance with the secondary antibody specification, at room temperature for 1 h, and the membrane was washed three times with TBST buffer for 20 min. The sample was then treated with ECL, and color development was carried out in the dark. After scanning, the optical density was analyzed by ImageJ, and the data were collected for statistical treatment using SPSS software. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Homo sapiens
Diseases Metabolic Diseases, Multiple Sclerosis, Atherosclerosis