Computational protocol: Genome-wide distribution comparative and composition analysis of the SSRs in Poaceae

Similar protocols

Protocol publication

[…] The SSR primers flanking 60 bp of the SSR loci were designed using eprimer3 []. Using the genome sequences of nine grass species, all the primers were tested and filtered by e-PCR []. The universal primers were selected using all the SSR primers amplified in the nine genomes, and then a polymorphism analysis was performed in silico on the genomes of Japonica, Indica and PA64. Some of the universal primers were also located on the genome using Mapdraw []. […]

Pipeline specifications

Software tools EMBOSS, e-PCR
Application qPCR
Organisms Homo sapiens
Diseases Ataxia Telangiectasia