Computational protocol: Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling

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Protocol publication

[…] Proliferation of cells was cytochemically detected according to the manufacturer’s instructions (C10310-3, Ribobio, Guangzhou, China). In brief, the tumor cells were incubated with the EdU staining buffer, fixed in 4% polyformaldehyde and stained the nuclear with DAPI. The stained cells were observed and photographed under microscope. In addition, integrated optical density (IOD) was used to assess EdU-positive cells with ImageJ software (Bethesda, MD, USA). [...] All the statistical analyses were performed with Sigmaplot software (Systat Software Inc., San Jose, CA, USA). The difference between the processed samples and the normal controls was examined using Student’s t-test. […]

Pipeline specifications

Software tools ImageJ, SigmaPlot
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Homo sapiens, Homo sapiens/Mus musculus xenograft, Mus musculus
Diseases Colonic Neoplasms, Neoplasms
Chemicals Curcumin, Progesterone