Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 3
Release date: Nov 17 2011
Last update date: Jul 26 2018
Access: Public
Diseases: Alzheimer Disease, Chromosome Disorders, Leukoencephalopathies
Dataset link Gene expression profile of THP-1 monocytes following knockdown of DAP12, a causative gene for Nasu-Hakola disease

Experimental Protocol


The siRNA vector construct targeting the DAP12 sequence (SI) and the control vector construct targeting the scrambled sequence (SCR) were generated by using GeneClip U1 Hairpin cloning system (Promega). The vectors were transfected in THP-1 cells by using Lipofectamine LTX reagent. The stable cell lines were selected by incubating them for approximately two months in the feeding medium with inclusion of 200 microgram/ml Hygromycin B. Then, two SI clones named SI5 and SI17, in addition to two SCR clones named SCR1 and SCR4, were selected by limiting dilution of the cells in a manner of a single cell per well plated in a 96-well cell culture plate.

Repositories


GEO

GSE33500

ArrayExpress

E-GEOD-33500

BioProject

PRJNA148849

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Contact


Jun-ichi Satoh