Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 71
Release date: Jan 12 2018
Last update date: Nov 21 2018
Access: Public
Dataset link Accurate H3K27 methylation patterns can be established de novo by SUZ12-directed PRC2

Experimental Protocol


H3K27me1, H3K27me2, H3K27me3 enrichment profiles (ChIPseq) analyzed in mouse ES cells which are wildtype, knockout for Ezh1 and Ezh2 (Ezh1/Ezh2 dKO), or re-expressing wildtype EZH2 (10 samples including 1 ChIP input sample for Ezh1/Ezh2 dKO). H3K27me1, H3K27me2, H3K27me3 enrichment profiles (ChIPseq) analyzed in mouse ES cells which are wildtype, knockout for Suz12, or re-expressing either wildtype SUZ12 or one of two SUZ12 fragment (15 samples). In addition, H3K27me1, H3K27me2, H3K27me3 enrichment profiles (ChIPseq) analyzed in E14 mouse ES cells treated with Ezh2 inhibitor and followed in a timecourse study after inhibitor washout (24 samples). PRC2 subunit enrichment profiles through Suz12, Ezh2 and Flag ChIPseq in various PRC2 wildtype, knockout or mutant ES cells (9 Suz12 ChIP samples, 2 Flag ChIP samples, 2 Ezh2 ChIP samples). Differential expression analysis (RNAseq) of mouse ES cells which are wildtype (3 replicates), knockout for Ezh1 and Ezh2 (Ezh1/Ezh2 dKO) (3 replicates), or re-expressing wildtype EZH2 (3 replicates) (9 samples).

Repositories


GEO

GSE103685

ENA

SRP117246

BioProject

PRJNA404057

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Contact


Jonas Hojfeldt