Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 72
Release date: Jun 17 2010
Last update date: Mar 22 2012
Access: Public
Diseases: Insulin Resistance
Genes: HSF1
Dataset link Insulin-like Signaling Determines Survival During Stress via Post Transcriptional Mechanisms in C. elegans.

Experimental Protocol


72 experimental samples were analyzed using custom oligo microarrays. A wild type sample pool was used as the Cy3 reference/control for all experimetal samples. All extracted RNA prior to array analysis was fractioned (via a sucrose gradient) based on ribosomal loading and pooled into ribosomal and free RNA (F1), light polysomes (F2) and heavy polysomes (F3) as described in the experimental procedures. The control RNAi is ‘empty’ vector L4440 RNAi feeding vector plasmid (1999 Firelab vector kit) transformed HT115(DE3), which was obtained from the Caenorhabditis Genetics Center (University of Wisconsin).

Repositories


GEO

GSE22383

ArrayExpress

E-GEOD-22383

BioProject

PRJNA127629

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Contact


Simon Melov