|Number of samples:||10|
|Release date:||Jul 24 2014|
|Last update date:||Oct 10 2018|
|Genes:||LCK, CASP3, USP3, Usp3, Hel1, MS4A1, ABCC3, ENG, TEK|
|Dataset link||Quantitative Analysis by Next Generation Sequencing of LSK (Lin- Sca1+ cKit+) hematopoietic progenitors and splenic B cells (naive and in vitro LPS activated) transcriptomes from Wild Type and Usp3-/- mice|
mRNA profiles of 8 weeks-old wild type (WT) and Usp3-/- mice were generated by deep sequencing, in duplicate, using Illumina Hiseq2000. For each experiment wt n=4, Usp3-/- n=4 mice were analized. FACS sorted cells from from individual animals were pooled and subjected to deep sequencing. Cells were: LSK (Lin- Sca1+ cKit+) from bone marrow, sorted naive B cells from spleens (CD19+) and activated B cells harvested and FACS sorted after 4 days stimulation with lipopolysaccharide (LPS) in culture.
Data in Brief
The Journal of Experimental Medicine