Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 10
Release date: Jul 24 2014
Last update date: Oct 10 2018
Access: Public
Genes: LCK, CASP3, USP3, Usp3, Hel1, MS4A1, ABCC3, ENG, TEK
Taxon: Mus musculus
Dataset link Quantitative Analysis by Next Generation Sequencing of LSK (Lin- Sca1+ cKit+) hematopoietic progenitors and splenic B cells (naive and in vitro LPS activated) transcriptomes from Wild Type and Usp3-/- mice

Experimental Protocol


mRNA profiles of 8 weeks-old wild type (WT) and Usp3-/- mice were generated by deep sequencing, in duplicate, using Illumina Hiseq2000. For each experiment wt n=4, Usp3-/- n=4 mice were analized. FACS sorted cells from from individual animals were pooled and subjected to deep sequencing. Cells were: LSK (Lin- Sca1+ cKit+) from bone marrow, sorted naive B cells from spleens (CD19+) and activated B cells harvested and FACS sorted after 4 days stimulation with lipopolysaccharide (LPS) in culture.

Repositories


GEO

GSE58495

ArrayExpress

E-GEOD-58495

ENA

SRP043219

BioProject

PRJNA252741

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